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荧光手电筒用于可卡因诱导精子甲基化

作者:上海峰志仪器 时间:2022-10-07 21:23:52浏览570 次

信息摘要:

2022年4月,宾夕法尼亚大学的研究人员在《Journal of Neuroscience》发表了《Cocaine-Induced Changes in Sperm Cdkn1a Methylation Are Associated with Cocaine Resistance in Male Offspring》,文献的实验使用了手持式双波长荧光蛋白观察手电筒观察绿色荧光蛋白GFP在小鼠身上的表达。手持式双波长荧光蛋白观察手电筒配合荧光观察眼镜能够快速地观察荧光蛋白在动物组织器官上的表达。

2022年4月,宾夕法尼亚大学的研究人员在《Journal of Neuroscience》发表了《Cocaine-Induced Changes in Sperm Cdkn1a Methylation Are Associated with Cocaine Resistance in Male Offspring》,文献的实验使用了手持式双波长荧光蛋白观察手电筒观察绿色荧光蛋白GFP在小鼠身上的表达。DFP-1手持式双波长荧光蛋白观察手电筒配合荧光观察眼镜能够快速地观察荧光蛋白在动物组织器官上的表达。

DFP-1手持式双波长荧光蛋白观察手电筒已经停产,如果您对手持式双波长荧光蛋白观察手电筒、GFP激发光源感兴趣,请参考美国路阳生产的LUYOR-3415RG双波长荧光蛋白观测灯,点击浏览:便携式荧光蛋白激发光源LUYOR-3415RG

文献摘要:

Virally infused rats were given 2.5-3.5 weeks after surgery to ensure knockdown or overexpression of Cdkn1a and expression of GFP control before initiation of cocaine (1.0 mg/kg/inf) self-administration as described above. Rats responded for cocaine on an FR1 schedule for 10 d. The following day, rats were switched to an FR5 schedule for 2 d and then to a PR schedule for 2 d. Under the PR schedule, the response requirement for subsequent drug delivery increased until the session was terminated 30 min after the last completed schedule. The response requirement for the “ith” reinforcement was R(1) = [5e°.2i-5] (Richardson and Roberts, 1996). The breakpoint was operationally defined as the total number of responses for the last completed schedule. Upon completion of behavioral experiments, catheter patency was confirmed by intravenous infusion of ketamine (0.1 ml). Twenty-four hours after the last self-administration session, rats were killed, viral placement was visualized using NIGHTSEA DFP Dual Fluorescent Protein Excitation Flashlight with NIGHTSEA Barrier Filter Glasses (Electron Microscopy Services), and the fluorescent region of the NAc was harvested.

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文献全文地址:https://doi.org/10.1523/JNEUROSCI.3172-20.2022   

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