经济实用的荧光检测GFP手电筒

经济实用的荧光检测GFP手电筒

产品名称：荧光蛋白检测双波长GFP手电筒

目录编号：DFP-GC

使用这款 DFP-GC LED GFP手电筒（便携式激发光源），可以轻松检测小鼠组织中的荧光蛋白表达。一个很好的便携式DFP-1 GFP手电筒能够替代昂贵的荧光立体显微镜。

实验设计和结果总结

应用：转基因小鼠筛选

起始材料：小鼠胚胎或解剖大脑

协议概述

1) 将鼠标或解剖的大脑放入开放容器中 2) 戴上特殊眼镜（根据荧光团选择） 3) 打开手电筒（开关允许在荧光团之间进行选择） 4) 关闭房间灯 5)观察

：对于高表达荧光团，不需要解剖也可用于新生小鼠，它可以代替基因分型。我们使用绿色和红色贴纸点来标记眼镜和开关，以便于使用。

总结

这是检查您的子宫内电穿孔大脑是否表达 RFP 或 GFP 的好方法。价格仅为荧光立体显微镜的一小部分。解剖后的大脑可以浸没在液体中，准备切片。电穿孔的点更亮并且明显受限。它看起来很小，因为您没有放大镜，我认为是因为您只能看到较亮的（中间）区域。您可以在工作台上快速检查并继续实验。此外，我们使用DFP-1 GFP手电筒在 P10 之前对表达 YFP 的小鼠进行基因分型。它可以装在箱子里运送到动物设施。

功能总结

您可以使用一个手电筒轻松筛选两种不同颜色的荧光团。

补充笔记

美国nightsea提供良好的技术支持，可帮助您在购买前决定过滤器/荧光观察眼镜

概括：

好处：以显微镜价格的一小部分进行快速筛选。可以通过EMS订购

缺点：过滤器不是很好。例如，在整个大脑中 GFP 高表达的小鼠中，您无法从子宫内电穿孔的载体中检测到 RFP 的表达。

总结：我们推荐这款GFP手电筒在神经科学实验室快速筛查荧光。

以上内容为机器翻译，仅供参考，以下面英文内容为准！

推荐阅读：便携式荧光蛋白激发光源LUYOR-3415RG 

上海峰志仪器有限公司代理销售美国nightsea、美国路阳便携式荧光蛋白手电筒，有单波长、双波长以及体视显微镜荧光适配器。具体优惠价格请按照网页底部的联系方式电话或微信联系。

荧光手电筒观察GFP的表达效果

荧光手电筒观察红色荧光蛋白的表达效果

Cheap and Practical Fluorescence Detector GFP Flashlight

Product Name:DFP Flashlight

Catalog Number:DFP-GC

We can easily detect fluorescent protein expression in mouse tissue with this LED flashlight. A great portable alternative to an expensive fluorescent stereomicroscope.

Experimental Design and Results Summary

Application

Transgenic mice screening

Starting Material

Mouse embryos or dissected brain

Protocol Overview

1) Place the mouse or dissected brain in an open container 2) Put on the special glasses (select depending on the fluorophore) 3) Turn on the flashlight (the switch allows to select between fluorophores) 4) Turn off the room lights 5) Observe

Tips

For high expression fluorophores no dissection is needed also used on newborn mice it can replace genotyping. We used green and red sticker dots to label glasses and switches for easy use.

Results Summary

This is a great way to check if your in-utero electroporated brains express RFP or GFP. At just a fraction of the price of a fluorescence stereo-microscope. The dissected brain can be submerged in liquid, ready for slicing. The electroporated spot is much brighter and clearly confined. it looks small because you don't have an amplification lens and I think because you only see the brighter (middle) region. You can check very fast over your bench and continue the experiment. In addition we use the flashlight to genotype YFP-expressing mice before P10. It can be transported in its case to the animal facility.

Features Summary

You can easily screen for two different colored fluorophores with one flashlight.

Additional Notes

Good tech support at nightsea.com to help you decide on filters/glasses beforr buying

Summary

The Good：Fast screening at a fraction of the price for a microscope. Can be ordered through EMS

The Bad：The filters are not very good. For example, in a mouse with high expression of GFP in all the brain you can't detect expression of RFP from a vector electroporated in-utero.

The Bottom Line：We would recommend this flashlight for fast screening of fluorescence in a neuroscience laboratory