sfGFP荧光蛋白的激发波长和发射波长

sfGFP荧光蛋白的激发波长和发射波长

sfGFP的激发波长主峰在488nm，发射波长主峰在507nm。

上海峰志仪器有限公司现货销售进口便携式荧光蛋白激发光源，又叫荧光蛋白观察灯、GFP激发光源，这个便携式光源能够激发GFP、EGFP、BFP、CFP、YFP、RFP、Dsred，mcherry等各种荧光蛋白的荧光，常备现货的有绿色荧光蛋白、红色荧光蛋白、黄色荧光蛋白激发光源咨，询电话：13818683556

常用的报告基因包括：lacZ、Egfp 、zsGreen、EYFP、DsRed (DsRed2)、mRFP、mCherry、HuCD2、Thy1.1、HA tag、FLAG tag、Luciferase等。zsGreen和EGFP, EYFP都是绿色荧光。EGFP和EYFP很难区分开。zsGreen是一个比较稳定的绿色荧光蛋白。DsRed和TdTomato都是红色荧光。TdTomato是一个信号非常强的荧光蛋白，对细胞/小鼠也没有明显的毒性。mRFP和mCherry是两个非常好的荧光蛋白，需要530nm的激发光，所以可以用LUYOR-3415RG荧光蛋白观察灯激发，能够用肉眼观察到荧光蛋白在小鼠上的表达。

上海峰志仪器有限公司销售美国路阳便携式荧光蛋白激发光源、荧光手电筒，能够激发各种荧光蛋白在动植物上的转基因表达，如果您要观察GFP荧光蛋白在植物叶片、愈伤、种子、根系的转基因表达。上海峰志提供样机免费试用，满意付款。我们还在北京、郑州、海口、武汉、哈尔滨设有代理商，可以提供上门演示。详情请按照网页底部电话或微信联系。

sfGFP--Superfolder GFP

Molecular engineering of the green fluorescent protein (GFP) into a robust and stable variant named Superfolder GFP (sfGFP) has revolutionized the field of biosensor development and the use of fluorescent markers in diverse area of biology. sfGFP-based self-associating bipartite split-FP systems have been widely exploited to monitor soluble expression in vitro, localization, and trafficking of proteins in cellulo. A more recent class of split-FP variants, named « tripartite » split-FP, that rely on the self-assembly of three GFP fragments, is particularly well suited for the detection of protein-protein interactions. In this review, we describe the different steps and evolutions that have led to the diversification of superfolder and split-FP reporter systems, and we report an update of their applications in various areas of biology, from structural biology to cell biology. 

What is Superfolder GFP?

Superfolder GFP is a basic (constitutively fluorescent) green fluorescent protein published in 2005, derived from Aequorea victoria. It is reported to be a very rapidly-maturing weak dimer. +.