荧光手电筒用于观察茜素红染色的海马骨架

荧光手电筒用于观察茜素红染色的海马骨架

用于骨架的染料是茜素红 S。它可以在白光下观察，但在适当波长的激发下它会发出美丽的红色。该图像是在配备体视显微镜荧光适配器的体视显微镜上拍摄的，使用绿色 (GR) 激发光源和匹配的红色屏障滤光片。

染红的骨骼会在荧光手电筒照射下发出荧光

Fluorescence of Cleared and Stained Specimens

We received this beautifully detailed fluorescence image of a seahorse skeleton from Kevin Conway at Texas A&M University. The specimen, a dwarf seahorse (Hippocampus zosterae), was prepared using a technique called ‘clearing and staining’, a method that produces an intact specimen with cartilage stained blue and bones stained red. It is a great way visualize the entire structure of an organism.

The stain used for the skeleton is Alizarin Red S. It can be viewed under white light, but under appropriate excitation it glows a beautiful red. The image was made on a stereo microscope outfitted with the Stereo Microscope Fluorescence Adapter, using the Green (GR) excitation source and matching red barrier filter.

染红的骨骼会在荧光手电筒照射下发出荧光

Morphological variation in support of the relationships among the Centropomoidei. Images of cleared and stained specimens fluorescing under royal blue light. Presence of rostral extension of external process on maxilla (character 361)—(A) Centropomus undecimalis (FMNH 77806), arrow, lateral view of right maxilla, scale bar = 5 mm; (B) Centropomus undecimalis (FMNH 77806), arrow, dorsal view of right maxilla, scale bar = 5 mm; (C) Sphyraena barracuda (FMNH 74209), arrow, lateral view of right maxilla, scale bar = 5 mm; (D) Sphyraena barracuda (FMNH 74209), arrow, dorsal view of right maxilla, scale bar = 5 mm. Enlargement of the second abdominal neural spine (character 1621)—(E) Lates calcarifer (AMNH 37839), arrow, right lateral view, scale bar = 1 mm; (F) Centropomus undecimalis (FMNH 77806), arrow, right lateral view, scale bar = 2.5 mm; (G) Lactarius lactarius (KUI 41405), arrow, right lateral view, scale bar = 5 mm. Possession of elongated, fang-like teeth in the oral jaws (character 261) and oral teeth that are ankylosed to the premaxilla and dentary (character 271)—(H) Sphyraena idiastes (SIO 15–182), lateral view of right premaxilla, scale bar = 1 mm; (I) Lactarius lactarius (KUI 41405), lateral view of right premaxilla, scale bar = 1 mm; (J) Lactarius lactarius (KUI 41405), lateral view of right dentary, scale bar = 1 mm.

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LUYOR-3415RG便携式荧光激发光源

一直以来，脊椎动物研究人员都靠所谓的清理和染色的方法（剥离标本的软组织，用红色染料染色），拍摄详细的图片，进行解剖检查，分析物种之间的关系。但由于失去了韧带和肌肉组织，骨骼松弛，很难支撑，科学家无法从特定角度进行拍摄。脊椎动物研究人员利用这些图像来探索动物的进化过程，并辨别它们与其他物种的共同特征。

如果没有组织，像白眼蟒这样的动物，很多骨骼会柔弱无骨，无法定型。但明胶能把它们固定住，拍完照片后再清洗掉。

在LUYOR-3280或LUYOR-3415荧光手电筒照射下，甘油明胶基质支撑的红色染料染过的海马骨骼发着光。这种摄影技术可以帮助科学家用一种新方法检查骨骼。